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药靶细胞株 > kinase激酶细胞株 > CBP73184Her2 A775_G776insYVMA/BaF3

Her2 A775_G776insYVMA/BaF3
名称 Her2 A775_G776insYVMA/BaF3
型号 CBP73184
报价
特点 Her2 A775_G776insYVMA/BaF3,母细胞:BaF3,冻存条件:90% FBS+10% DMSO,培养基:RPMI-1640+10%FBS+1 ug/ml puromycin
  • 详细内容
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CBP73184
I. Introduction

Cell Line Name:

Her2 A775_G776insYVMA/BaF3

Gene Synonyms:

NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu

Host Cell:

Ba/F3

Stability:16 passages

Application:

Anti-proliferation assay and PD assay

Freeze Medium:

90% FBS+10% DMSO

Complete Culture Medium:

RPMI-1640+10%FBS+1 ug/ml puromycin

Mycoplasma Status:

Negative

 
II.Background

ERBB2 (HER2) A775_G776insYVMA results in the insertion of four amino acids in the protein kinase domain of the Erbb2 (Her2) protein between amino acids 775 and 776 (UniProt.org). A775_G776insYVMA results in the same amino acid change as M774_A775insAYVM, which leads to constitutive phosphorylation of Erbb2 (Her2) and is transforming in cell culture (PMID: 17311002, PMID: 29967253).

 
III. Representative Data

1. WB of Her2 A775_G776insYVMA/BaF3

CBP73184 WB.png

 

2. Anti-proliferation assay

CBP73184 fig.png

Figure 2. Anti-proliferation assay of two reference compounds on the Her2 A775_G776insYVMA/BaF3 Stable Cell Line


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