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药靶细胞株 > 免疫治疗细胞株 > CBP74034CD40/CHO

CD40/CHO
名称 CD40/CHO
型号 CBP74034
报价
特点 CD40/CHO,冻存条件:90% FBS+10% DMSO,培养基:F12k+10%FBS+5ug/ml puromycin
  • 详细内容

CD40/CHO

CBP74034

I. Background
CD40, a TNF receptor superfamily member, was initially identified on B lymphocytes. However, other cell types such as monocytes, basophils, dendritic cells, endothelial cells, and epithelial cells have been found to express CD40. High levels of CD40 have also been detected in numerous human cancers, including HCT116, a colon cancer epithelial cell line. Interaction with CD40 ligand (CD40L, CD154) on CD4+ T helper lymphocytes triggers the expression of intercellular adhesion molecule (ICAM) and other pro-inflammatory cytokines. CD40:CD40L signaling simultaneously increases activation of antigen-specific T cells. CD40 also activates NF-κB-dependent signaling in response to lipopolysaccharide (LPS) found on Gram negative bacterial pathogens. Agonistic CD40 monoclonal antibodies have been shown to activate antigen presenting cells (APC), promote anti-tumor T-cell responses, and to foster cytotoxic myeloid cells, suggesting a potential mechanism to control cancer in the absence of T-cell immunity.

II. Description

Recombinant CHO cell line expressing full length human CD40 (Tumor necrosis factor receptor superfamily member 5; TNFRSF5), Genbank Accession No. NP_001241.1. Expression is confirmed by real-time qPCR and Western blot.


III. Introduction
Host Cell:CHO
Expressed gene:CD40
Stability:32 passages
Freeze Medium:90% FBS+10% DMSO
Culture Medium:

F12k+10%FBS+5ug/ml puromycin

Application(s):Since HEK293 cell line does not express endogenous CD40, the CD40 HEK293 Cell Line is ideal to monitor CD40-specific signaling responses, such as NF-κB activation in response to potential CD40 agonistic monoclonal antibodies.
Storage:Liquid nitrogen

IV. Description of Host Cell Line
Organism:Cricetulus griseus, hamster, Chinese
Tissue:Ovary
Disease: Hamster Chinese ovary
Morphology: Epitheloid cell
Growth Properties: Adherent

Ⅴ. Representative Data

CBP74034.png

Figure 1. Recombinant CHO cell line expressing full length human CD40 (Tumor necrosis factor receptor superfamily member 5; TNFRSF5), Genbank Accession No. NP_001241.1. Expression is confirmed by real-time qPCR and Western blot.

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