| 名称 | CD40/CHO |
| 型号 | CBP74034 |
| 报价 |  |
| 特点 | CD40/CHO，冻存条件：90% FBS+10% DMSO |
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电话：86-25-86880024
号码：86-25-86880024
手机：18066071954
地址：南京市雨花区长虹路222号
Email： duhaitao@cobioer.com
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- 详细内容
| CBP74034 | |
| I. Background | |
| CD40, a TNF receptor superfamily member, was initially identified on B lymphocytes. However, other cell types such as monocytes, basophils, dendritic cells, endothelial cells, and epithelial cells have been found to express CD40. High levels of CD40 have also been detected in numerous human cancers, including HCT116, a colon cancer epithelial cell line. Interaction with CD40 ligand (CD40L, CD154) on CD4+ T helper lymphocytes triggers the expression of intercellular adhesion molecule (ICAM) and other pro-inflammatory cytokines. CD40:CD40L signaling simultaneously increases activation of antigen-specific T cells. CD40 also activates NF-κB-dependent signaling in response to lipopolysaccharide (LPS) found on Gram negative bacterial pathogens. Agonistic CD40 monoclonal antibodies have been shown to activate antigen presenting cells (APC), promote anti-tumor T-cell responses, and to foster cytotoxic myeloid cells, suggesting a potential mechanism to control cancer in the absence of T-cell immunity. | |
| II. Description | |
Recombinant CHO cell line expressing full length human CD40 (Tumor necrosis factor receptor superfamily member 5; TNFRSF5), Genbank Accession No. NP_001241.1. Expression is confirmed by real-time qPCR and Western blot. | |
| III. Introduction | |
| Host Cell: | CHO |
| Expressed gene: | CD40 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12k+10%FBS+5ug/ml puromycin |
| Application(s): | Since HEK293 cell line does not express endogenous CD40, the CD40 HEK293 Cell Line is ideal to monitor CD40-specific signaling responses, such as NF-κB activation in response to potential CD40 agonistic monoclonal antibodies. |
| Storage: | Liquid nitrogen |
| IV. Description of Host Cell Line | |
| Organism: | Cricetulus griseus, hamster, Chinese |
| Tissue: | Ovary |
| Disease: | Hamster Chinese ovary |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| Ⅴ. Representative Data | |
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Figure 1. Recombinant CHO cell line expressing full length human CD40. | |
