| 名称 | CD40/NFkB-Luc/HEK293 |
| 型号 | CBP74009 |
| 报价 |  |
| 特点 | CD40/NFkB-Luc/HEK293，冻存条件：90% FBS+10% DMSO |
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电话：86-25-86880024
号码：86-25-86880024
手机：18066071954
地址：南京市雨花区长虹路222号
Email： duhaitao@cobioer.com
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- 详细内容
| CBP74009 | |
| I. Background | |
| CD40, a TNF receptor superfamily member, was initially identified on B lymphocytes. However, antigen presenting cells (APCs) such as monocytes, basophils, dendritic cells and non-immune cells like endothelial cells and epithelial cells have been found to express CD40. A wide variety of carcinoma cells also over-express CD40. Interaction with CD40 ligand (CD40L, CD154) on CD4+ T helper lymphocytes triggers the expression of intercellular adhesion molecule (ICAM) and other pro-inflammatory cytokines. CD40:CD40L signaling simultaneously increases activation of antigen-specific T cells. CD40 also activates NF-κB-dependent signaling in response to lipopolysaccharide (LPS) found on Gram negative bacterial pathogens. Furthermore, agonistic CD40 monoclonal antibodies have been shown to activate antigen presenting cells (APC) and promote anti-tumor T-cell responses in addition to fostering cytotoxic myeloid cells with the potential to control cancer in the absence of T-cell immunity. | |
| II. Description | |
Recombinant HEK293 cell line expressing full length human CD40 (Tumor necrosis factor receptor superfamily member 5; TNFRSF5). Expression is confirmed by real-time qPCR and western blot. This NF-ĸB luciferase reporter construct is stably integrated into the genome. The firefly luciferase gene is controlled by 4 copies of NF-kB response element located upstream of the TATA promoter. Following activation by human CD40 ligand, NF-ĸB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene. | |
| III. Introduction | |
| Host Cell: | HEK293 |
| Expressed gene: | CD40 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | NF-κB cell line, NFkappaB cell line, luciferase reporter, NF-kB cell line, NFkB cell line, CD40 cell line, cd40 luciferase reporter, CD40 HEK293 cell line |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | MEM + 10% FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP)+1ug/ml puromycin+100ug/ml hygromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | The CD40 NF-ĸB Reporter HEK293 Stable Cell Line is ideal for High Throughput Screening (HTS) to identify potential CD40 agonistic monoclonal antibodies and CD40-specific inhibitors. |
| IV. Description of Host Cell Line | |
| Organism: | Human |
| Tissue: | kidney |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| Ⅴ. Representative Data | |
Figure 1. Recombinant HEK293 cell line expressing firefly luciferase gene under the control of NFκB response elements with constitutive expression of CD40. | |
Figure 2. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. HEK293-CD40-NFκB Luciferase Reporter cells were stimulated by CD40L, the EC50 was 43ng/ml. | |
