STAT3-Luc/HEK293-南京科佰生物科技有限公司

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Email： zhangxiangwen@cobioer.com

STAT3-Luc/HEK293

.CBPB0002
I. Background
The STAT3 Reporter (Luc)-HEK293 cell line is designed for monitoring STAT3 signal transduction pathway. It contains a firefly luciferase gene driven by STAT3 response elements located upstream of the minimal TATA promoter.

II. Description
After activation by cytokines and growth factors, endogenous STAT3 binds to the DNA response elements, inducing transcription of the luciferase reporter gene.

III. Introduction
Host Cell:	HEK293
Expressed gene:	STAT3-Luciferase
Stability:	32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s):	Acute-Phase Response Factor, DNA-Binding Protein APRF, DNA, ADMIO, Signal Transducer And Activator Of Transcription 3, TATA
Freeze Medium:	90% FBS+10% DMSO
Culture Medium:	DMEM + 10%FBS+100ug/ml hygromycin
Mycoplasma Testing:	Negative
Storage:	Liquid nitrogen

IV. Description of Host Cell Line
Organism:	Homo sapiens, human
Tissue:	Embryonic kidney
Disease:	Normal
Morphology:	Epitheloid
Growth Properties:	Adherent

Ⅴ. Representative Data

Figure 1. Inhibition of IL-6-induced Reporter Activity by IL-6 Neutralizing Antibody in STAT3 (Luc) Reporter HEK293 Cells.
Figure 2.Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). STAT3 Luciferase Reporter-HEK293 Cells (C7).