名称 | STAT3-Luc/HEK293 |
型号 | .CBPB0002 |
报价 | |
特点 | STAT3-Luc/HEK293,冻存条件:90% FBS+10% DMSO |
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电话:4008750250
号码:
手机:18066071954
地址:南京市栖霞区纬地路9号
Email: zhangxiangwen@cobioer.com
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- 详细内容
STAT3-Luc/HEK293
.CBPB0002 | |
I. Background | |
The STAT3 Reporter (Luc)-HEK293 cell line is designed for monitoring STAT3 signal transduction pathway. It contains a firefly luciferase gene driven by STAT3 response elements located upstream of the minimal TATA promoter. | |
II. Description | |
After activation by cytokines and growth factors, endogenous STAT3 binds to the DNA response elements, inducing transcription of the luciferase reporter gene. | |
III. Introduction | |
Host Cell: | HEK293 |
Expressed gene: | STAT3-Luciferase |
Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Synonym(s): | Acute-Phase Response Factor, DNA-Binding Protein APRF, DNA, ADMIO, Signal Transducer And Activator Of Transcription 3, TATA |
Freeze Medium: | 90% FBS+10% DMSO |
Culture Medium: | DMEM + 10%FBS+100ug/ml hygromycin |
Mycoplasma Testing: | Negative |
Storage: | Liquid nitrogen |
IV. Description of Host Cell Line | |
Organism: | Homo sapiens, human |
Tissue: | Embryonic kidney |
Disease: | Normal |
Morphology: | Epitheloid |
Growth Properties: | Adherent |
Ⅴ. Representative Data | |
Figure 1. Inhibition of IL-6-induced Reporter Activity by IL-6 Neutralizing Antibody in STAT3 (Luc) Reporter HEK293 Cells. | |
Figure 2.Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly suggest to purchase from Cobioer). STAT3 Luciferase Reporter-HEK293 Cells (C7). |