STAT5-Luc/BaF3-南京科佰生物科技有限公司

产品搜索

联系人：蒋经理
电话：86-25-86880024
号码：86-25-86880024
手机：18066071954
地址：南京市雨花区长虹路222号
Email： zhangxiangwen@cobioer.com

STAT5-Luc/BaF3

.CBPB0001
I. Background
After activation by cytokines or growth factors, endogenous STAT5 binds to the DNA response elements, inducing transcription of the luciferase reporter gene.

II. Description
The STAT5 Reporter (Luc)-Ba/F3 cell line is designed for monitoring STAT5 signal transduction pathways. It contains a firefly luciferase gene driven by the STAT5 response element located upstream of the minimal TATA promoter.

III. Introduction
Expressed gene:	STAT5
Stability:	32 passages
Freeze Medium:	90% FBS+10% DMSO
Culture Medium:	RPMI-1640+10%FBS+10ng/ml IL-3+200ug/ml hygromycin
Mycoplasma Testing:	Negative
Storage:	Liquid nitrogen


IV. Representative Data
Figure 1.Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). STAT5 Luciferase Reporter - BaF3 Cells (C18)，the EC50 was 0.23ng/ml.