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药靶细胞株 > 肿瘤免疫细胞株 > CBP74031NFkB-Luc/Jurkat

NFkB-Luc/Jurkat
名称 NFkB-Luc/Jurkat
型号 CBP74031
报价
特点 NFkB-Luc/Jurkat,冻存条件:90% FBS+10% DMSO;
  • 详细内容
CBP74031
I. Description
NF-kB luciferase reporter construct is stably integrated into the genome of Jurkat T- cells. The firefly luciferase gene is controlled by 4 copies of NF-kB response element located upstream of the TATA promoter. Following activation by stimulants, endogenous NF-kB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene.

II. Introduction
Host Cell:Jurkat
Expressed gene:NF-kB Luciferase
Stability:32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s):Nfkb, nf-kb reporter, nfkb report, nf-kb jurkat
Freeze Medium:90% FBS+10% DMSO
Culture Medium:RPMI-1640+10%FBS+800ug/ml hygromycin
Mycoplasma Testing:Negative
Storage:Liquid nitrogen
Application(s):Functional(Report Gene) Assay

III. Description of Host Cell Line
Organism:Homo sapiens, human
Tissue:Peripheral blood
Disease: Acute T cell leukemia
Morphology: Lymphoblast
Growth Properties: Suspension

IV. Representative Data

image.png

Figure 1. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly suggest to purchase from Cobioer). Induction of NFkB activity by PMA with lonomycin in Jukart-NFkB Luciferase Reporter Cell.






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