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药靶细胞株 > 肿瘤免疫细胞株 > CBP74031NFkB-Luc/Jurkat

NFkB-Luc/Jurkat
名称 NFkB-Luc/Jurkat
型号 CBP74031
报价
特点 NFkB-Luc/Jurkat,冻存条件:90% FBS+10% DMSO;
  • 详细内容
CBP74031
I. Description
NF-kB luciferase reporter construct is stably integrated into the genome of Jurkat T- cells. The firefly luciferase gene is controlled by 4 copies of NF-kB response element located upstream of the TATA promoter. Following activation by stimulants, endogenous NF-kB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene.

II. Introduction
Host Cell:Jurkat
Expressed gene:NF-kB Luciferase
Stability:32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s):Nfkb, nf-kb reporter, nfkb report, nf-kb jurkat
Freeze Medium:90% FBS+10% DMSO
Culture Medium:RPMI-1640+10%FBS+800ug/ml hygromycin
Mycoplasma Testing:Negative
Storage:Liquid nitrogen
Application(s):The NF-kB-luciferase/Jurkat cell line is suitable for monitoring the activity of NF-kB signaling in response to stimulants, and establishing cell-based screens for inhibitors that target specific NF- kB-stimulating molecules.

III. Description of Host Cell Line
Organism:Homo sapiens, human
Tissue:Peripheral blood
Disease: Acute T cell leukemia
Morphology: Lymphoblast
Growth Properties: Suspension

IV. Representative Data

image.png

Figure 1. Induction of NFkB activity by PMA with lonomycin in Jukart-NFkB Luciferase Reporter Cell.




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