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药靶细胞株 > 免疫治疗细胞株 > CBP7404541BB/NFkB-Luc/HEK293

41BB/NFkB-Luc/HEK293
名称 41BB/NFkB-Luc/HEK293
型号 CBP74045
报价
特点 41BB/NFkB-Luc/HEK293,母细胞:HEK293,培养基:MEM + 10% Foetal Bovine Serum (FBS)+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) +1ug/ml puromycin+100ug/ml hygromycin
  • 详细内容

 

CBP74045
I. Background

Human CD137 (4-1BB; TNFRS9) is an inducible costimulatory molecule that activates T cells. CD137-CD137L mediated signaling has been shown to be important for proliferation, effector functions and survival of T cells. CD137 is also expressed in NK and NKT cells. Antibodies targeting CD137 activation in immune cells have demonstrated potent anti-tumor effects in cancer patients.

 
II. Description

Recombinant HEK293 cell constitutively expressing full length human CD137 (Genbank #NM_003811). Surface expression is confirmed by flow cytometry.

 
III. Introduction
Host Cell:HEK293
Expressed gene:

4-1BB

Stability:32 passages
Synonym(s):4-1BB, TNFRS9, Mitogen-Activated Protein Kinase 14, Signal Transducer And Activator Of Transcription 3, STAT3, MAPK14
Freeze Medium:90% FBS+10% DMSO
Culture Medium:

MEM + 10% Foetal Bovine Serum (FBS)+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) +1ug/ml puromycin+100ug/ml hygromycin

Mycoplasma Testing:Negative
Storage:Liquid nitrogen
Application(s):

Screen for activators or inhibitors of CD137 signaling in a cellular context and characterize the biological activity of CD137 and its interactions with ligands

 
IV. Description of Host Cell Line
Organism:Human
Tissue:Embryonic kidney
Disease: Normal
Morphology: Epitheloid
Growth Properties: Adherent
 
Ⅴ. Representative Data

CBP74045 fig1.png

Figure 1Recombinant HEK293 cell line expressing firefly luciferase gene under the control of NFκB response elements with constitutive expression of 4-1BB.

Figure 2. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. 4-1BB/NF-κB Reporter-HEK293 cells were stimulated by 4-1BBL, the S/B was 14.1-fold.


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