CD155/TCR Activator/CHO-南京科佰生物科技有限公司

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药靶细胞株 > 免疫治疗细胞株 > CBP74073CD155/TCR Activator/CHO

名称	CD155/TCR Activator/CHO
型号	CBP74073
报价
特点	CD155/TCR Activator/CHO，冻存条件：90% FBS+10% DMSO,培养基：1640+10%FBS+800ug/ml hygromycin+1ug/ml puromycin

详细内容

CBP74073
I. Background
TIGIT is a co-inhibitory receptor that is highly expressed in Natural Killer (NK) cells, activated CD4+, CD8+ and regulatory T cells. Interaction with the poliovirus receptor (PVR; CD155) on antigen presenting cells, such as dendritic cells, recruits Src homology (SH) domain-containing protein tyrosine phosphatase SHP1 and SHP2 or the inositol phosphatase SHIP1 and SHIP2 to the TIGIT ITIM domain. This increases IL-10 release and suppresses NF-kB and NFAT T cell receptor (TCR) signaling, which blocks T cell proliferation and cytokine production. It serves as a competitive inhibitor of CD226, a co-stimulatory receptor for CD155. TIGIT targeting antibodies that block this T cell-intrinsic inhibitory effects have shown enhanced anti-tumor and anti-viral functions in preclinical studies.

II. Description
Recombinant CHO-K1 cells constitutively expressing human CD155 (Poliovirus receptor, Nectin-like protein 5, GenBank accession # NM_006505) and an engineered T cell receptor (TCR) activator.

III. Introduction
Host Cell:	CHO
Expressed gene:	CD155
Stability:	32 passages
Synonym(s):	Poliovirus receptor, Nectin-like protein 5, CD155-His
Freeze Medium:	90% FBS+10% DMSO
Culture Medium:	1640+10%FBS+800ug/ml hygromycin+1ug/ml puromycin
Mycoplasma Testing:	Negative
Storage:	Liquid nitrogen
Application(s):	Screen for activators or inhibitors of TIGIT signaling in a cellular context, screen CD155 antibodies for binding affinity, and characterize the biological activity of TIGIT interactions with CD155.

IV. Description of Host Cell Line
Organism:	Cricetulus griseus, hamster, Chinese
Tissue:	Ovary
Disease:	Hamster Chinese ovary
Morphology:	Epitheloid cell
Growth Properties:	Adherent

Ⅴ. Representative Data
Figure 1.Recombinant CHO-cells constitutively expressing CD155 and an engineered T cell receptor (TCR) activator.
Figure 2. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. CHO-CD155-TCR activator Reporter cells were stimulated by CD155, the EC50 was 1.24μg/ml.