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药靶细胞株 > 免疫治疗细胞株 > CBP74010CSF1R/SRE-Luc/HEK293

CSF1R/SRE-Luc/HEK293
名称 CSF1R/SRE-Luc/HEK293
型号 CBP74010
报价
特点 CSF1R/SRE-Luc/HEK293,冻存条件:90% FBS+10% DMSO,培养基:DMEM +10%FBS+200ug/ml hygromycin+1ug/ml puromycin
  • 详细内容

 

CBP74010
I. Background

Colony Stimulating Factor 1 Receptor (CSF1R, CD115, M-CSF-R) is the receptor for the cytokine CSF1 (MCSF; CSF-1), which controls the growth, function, and differentiation of macrophages. This interaction drives the development and survival of Tumor-Associated Macrophages (TAMs), which in turn suppress the local immune response to the cancer. An alternative ligand is IL-34, which has been implicated in neuroprotective and survival signals following brain injury and neurodegeneration.

As a tyrosine kinase transmembrane receptor, activation of CSF1R by its ligand initiates a vast array of intracellular activity, including activation of the MAPK/ERK signaling pathway. When phosphorylated by ERK, Elk1 forms a complex with Serum Response Factor (SRF) and binds to Serum Response Element (SRE), resulting in the expression of numerous mitogen-inducible genes.

 
Ⅱ. Introduction
Host Cell:HEK293
Expressed gene:

CFS1R-SRE-Luciferase

Stability:32 passages
Synonym(s):FMS, CD115
Freeze Medium:90% FBS+10% DMSO
Culture Medium:

DMEM +10%FBS+200ug/ml hygromycin+1ug/ml puromycin

Mycoplasma Testing:Negative
Storage:Liquid nitrogen
Application(s):Screen for activators or inhibitors of the CSF1R/MAPK/ERK signaling pathway.
 
Ⅲ. Description of Host Cell Line
Organism:Human
Tissue:Embryonic kidney
Morphology: Epitheloid cell
Growth Properties: Adherent
 
Ⅳ. Representative Data

CBP74010-1.png

Figure 1. Recombinant HEK293 cell line expressing firefly luciferase gene under the control of CRE response elements with constitutive expression of CSF1R(Colony Stimulating Factor 1 Receptor)

CBP74010-2.png

Figure 2. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. HEK293/CFS1R-SRE Luciferase Reporter cells were stimulated by CSF1, the EC50 was 3.75ng/ml.

 

CBP74010-3.png

 Figure 3. HEK293/CFS1R-SRE Luciferase Reporter cells were stimulated by CSF1 inhibitors.


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