| 名称 | FOXP3 promoter-Luc/Jurkat |
| 型号 | CBP74011 |
| 报价 |  |
| 特点 | FOXP3 promoter-Luc/Jurkat，冻存条件：90% FBS+10% DMSO； |
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电话：86-25-86880024
号码：86-25-86880024
手机：18066071954
地址：南京市雨花区长虹路222号
Email： duhaitao@cobioer.com
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- 详细内容
| CBP74011 | |
| I. Background | |
| Foxp3, belonging to the forkhead family, is a master transcription factor that expresses exclusively in regulatory T cells, a subset of CD4+ T cells. Regulation of Foxp3 is critical for maintaining immunological tolerance. Over-expression of Foxp3 is known to suppress effector T cell activation. | |
| II. Description | |
Human Foxp3 luciferase reporter construct is stably integrated into the genome of Jurkat T- cells. The firefly luciferase gene is controlled by a human Foxp3 promoter and an enhancer-like conserved noncoding sequence upstream of the Foxp3 promoter.
Figure 1. Illustration of Foxp3 promoter region with representative transcription factor binding sites and enhancer regions. | |
| III. Introduction | |
| Host Cell: | Jurkat |
| Expressed gene: | Foxp3-Luciferase |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | Foxp3, Foxp3 cell line, scurfin cell line, foxo |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+1 ug/ml puromucin |
| Storage: | Liquid nitrogen |
| Application(s): | The Foxp3 promoter Jurkat reporter cell line is suitable for monitoring the transcription activity of Foxp3 in response to stimulant, and establishing cell-based screens for inhibitors that target specific Foxp3-stimulating molecules. |
| IV. Description of Host Cell Line | |
| Organism: | Homo sapiens, human |
| Tissue: | Peripheral blood |
| Disease: | Acute T cell leukemia |
| Morphology: | Lymphoblast |
| Growth Properties: | Suspension |
| Ⅴ. Representative Data | |
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Figure 2. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System, the EC50 was 0.21 ng/ml. | |
