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药靶细胞株 > 肿瘤免疫细胞株 > CBP74012GITR/NFκB-Luc/Jurkat

GITR/NFκB-Luc/Jurkat
名称 GITR/NFκB-Luc/Jurkat
型号 CBP74012
报价
特点 GITR/NFκB-Luc/Jurkat,冻存条件:90% FBS+10% DMSO;
  • 详细内容


CBP74012
I. Background

Glucocorticoid-induced TNFR-related protein (GITR) is a member of the TNFR superfamily, expressed in a number of cells including T cells, natural killer (NK) cells and antigen-presenting cells (APC). GITRL, GITR’s natural ligand, is expressed mainly by APCs and GITR:GITRL interaction is important for activation of the immune system. Agonistic antibodies targeting GITR are actively being pursued as potential immuno-oncology therapies.


II. Description
This cell line expresses a surface human GITR (glucocorticoid-induced TNFR family related gene; TNFRSF18; CD357) and an NF-kB luciferase reporter construct that are stably integrated into the genome of Jurkat T-cells.

III. Introduction
Host Cell:Jurkat
Expressed gene:

GITR-NFκB-Luciferase

Stability:32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium:90% FBS+10% DMSO
Culture Medium:

RPMI-1640+10%FBS+400ug/ml hygromycin+1ug/ml puromycin

Mycoplasma Testing:Negative
Storage:Liquid nitrogen
Application(s):

Functional(Report Gene) Assay


IV. Description of Host Cell Line
Organism:Human
Tissue:Peripheral blood
Disease: Childhood T acute lymphoblastic leukemia
Morphology: Lymphoblast
Growth Properties: Suspension

Ⅴ. Representative Data
 COBIOER

Figure 1. Recombinant Jurkat stably expressing human GITR.


 COBIOER


Figure 2.  Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly suggest to purchase from Cobioer). Jurkat/NF-κB Luciferase-GITR Reporter cells were stimulated by GITRL/CHO cells, the EC50 was 2634cells.


 COBIOER

Figure 3.Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly suggest to purchase from Cobioer).Jurkat/NF-κB Luciferase-GITR Reporter cells were stimulated by hGITRL, the EC50 was 0.15ug/ml.






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