名称 | GITR/NFκB-Luc/Jurkat |
型号 | CBP74012 |
报价 | |
特点 | GITR/NFκB-Luc/Jurkat,冻存条件:90% FBS+10% DMSO; |
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CBP74012 | |
I. Background | |
Glucocorticoid-induced TNFR-related protein (GITR) is a member of the TNFR superfamily, expressed in a number of cells including T cells, natural killer (NK) cells and antigen-presenting cells (APC). GITRL, GITR’s natural ligand, is expressed mainly by APCs and GITR:GITRL interaction is important for activation of the immune system. Agonistic antibodies targeting GITR are actively being pursued as potential immuno-oncology therapies. | |
II. Description | |
This cell line expresses a surface human GITR (glucocorticoid-induced TNFR family related gene; TNFRSF18; CD357) and an NF-kB luciferase reporter construct that are stably integrated into the genome of Jurkat T-cells. | |
III. Introduction | |
Host Cell: | Jurkat |
Expressed gene: | GITR-NFκB-Luciferase |
Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Freeze Medium: | 90% FBS+10% DMSO |
Culture Medium: | RPMI-1640+10%FBS+400ug/ml hygromycin+1ug/ml puromycin |
Mycoplasma Testing: | Negative |
Storage: | Liquid nitrogen |
Application(s): | Functional(Report Gene) Assay |
IV. Description of Host Cell Line | |
Organism: | Human |
Tissue: | Peripheral blood |
Disease: | Childhood T acute lymphoblastic leukemia |
Morphology: | Lymphoblast |
Growth Properties: | Suspension |
Ⅴ. Representative Data | |
Figure 1. Recombinant Jurkat stably expressing human GITR. |
Figure 2. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly suggest to purchase from Cobioer). Jurkat/NF-κB Luciferase-GITR Reporter cells were stimulated by GITRL/CHO cells, the EC50 was 2634cells. | |
Figure 3.Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly suggest to purchase from Cobioer).Jurkat/NF-κB Luciferase-GITR Reporter cells were stimulated by hGITRL, the EC50 was 0.15ug/ml. |