名称 | HVEM/NFκB-Luc/Jurkat |
型号 | CBP74013 |
报价 | ![]() |
特点 | HVEM/NFκB-Luc/Jurkat,冻存条件:90% FBS+10% DMSO; |
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Email: zhangxiangwen@cobioer.com
电话:4008750250
号码:
手机:18066071954
地址:南京市栖霞区纬地路9号
Email: zhangxiangwen@cobioer.com
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- 详细内容
I. Background | |
疱疹病毒进入介质(HVEM),也称为肿瘤坏死因子受体超家族成员 14 (TNFRSF14),是 TNF 受体(肿瘤坏死因子)超家族的人细胞表面受体。HVEM 在多种 免疫细胞中表达,包括 T 细胞、B 细胞、自然杀伤细胞、树突状细胞和内皮细胞。它还通 过在黑色素瘤细胞上的频繁表达参与肿瘤的免疫逃避。 | |
II. Description | |
HVEM NFκB-Luc Jurkat 报告基因药靶模型很好的模拟了体内 HVEM 的信号转导过程, 原理见下图所示。![]() Figure 1. HVEM NFκB-Luc Jurkat 细胞模型原理图 | |
III. Introduction | |
Host Cell: | Jurkat |
Expressed gene: | HVEM-NFκB-Luciferase |
Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Synonym(s): | HVEM, TNFRSF14, CD270, HVEA, TR2, LIGHTR, HVEM Cell line, HVEM NFAT |
Freeze Medium: | 90% FBS+10% DMSO |
Culture Medium: | RPMI-1640+10%FBS+800μg/ml hygromycin+1μg/ml puromycin |
Mycoplasma Testing: | Negative |
Storage: | Liquid nitrogen |
Application(s): | Functional(Report Gene) Assay |
IV. Description of Host Cell Line | |
Organism: | Human |
Tissue: | Peripheral blood |
Disease: | Childhood T acute lymphoblastic leukemia |
Morphology: | Lymphoblast |
Growth Properties: | Suspension |
Ⅴ. Representative Data | |
Figure 2. Recombinant Jurkat stably expressing human HVEM. |
Figure 3. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly suggest to purchase from Cobioer). HVEM NFκB-Luc Jurkat(C7) Report Assay Stimulated by LIGHT/CHO.
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