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药靶细胞株 > 肿瘤免疫细胞株 > CBP74014IL2_promoter-Luc/Jurkat

IL2_promoter-Luc/Jurkat
名称 IL2_promoter-Luc/Jurkat
型号 CBP74014
报价
特点 IL2_promoter-Luc/Jurkat,冻存条件:90% FBS+10% DMSO;
  • 详细内容
CBP74014
I. Background
The particles contain a firefly luciferase gene driven by the human IL-2 promoter (Figure 1). After transduction, activation of the IL-2 signaling pathway in the target cells can be monitored by measuring the luciferase activity.
II. Description
The IL-2 Promoter Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells.
III. Introduction
Host Cell:Jurkat
Expressed gene:IL-2-Luciferase
Stability:32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s):IL-2 Promoter reporter, lentivirus reporter, HIV reporter, VSV-G reporter
Freeze Medium:90% FBS+10% DMSO
Culture Medium:RPMI-1640+10%FBS+1ug/ml puromycin
Mycoplasma Testing:Negative
Storage:Liquid nitrogen
Application(s):• Screen for activators or inhibitors of IL-2 signaling pathway in transduced target cells

• Generation of IL-2 promoter luciferase reporter stable cell line

IV. Description of Host Cell Line
Organism:Homo sapiens, human
Tissue:Peripheral blood
Disease: Acute T cell leukemia
Morphology: Lymphoblast
Growth Properties: Suspension
Ⅴ. Representative Data


 COBIOER

Figure 1. Response of IL -2-Luciferase Reporter Jurkat Cell Line (C29) to Raji TCR Activator Cell Line.


 COBIOER

Figure 2. Dose Response of Recombinant hCTLA4-Fc in IL2 Promoter Reporter-Jurkat Cells with TCR Activitor Raji Cells.



 COBIOER

Figure 3. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. Jurkat-IL-2 Luciferase Reporter cells were stimulated by anti-CD3, the EC50 was 8.31ng/ml.



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