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药靶细胞株 > 肿瘤免疫细胞株 > CBP74017OX40/NFkB-Luc/HEK293

OX40/NFkB-Luc/HEK293
名称 OX40/NFkB-Luc/HEK293
型号 CBP74017
报价
特点 OX40/NFkB-Luc/HEK293,冻存条件:90% FBS+10% DMSO;
  • 详细内容

OX40/NFkB-Luc/HEK293

CBP74017
I. Background
Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40, is a member of the TNFR-superfamily of receptors. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation. This receptor has been shown to activate NF-kappaB through its interaction with adaptor proteins TRAF2 and TRAF5. Its ligand, OX40L, binds to OX40 receptors on T-cells, preventing them from dying and subsequently increasing cytokine production. OX40 has a critical role in the maintenance of an immune response beyond the first few days and onwards to a memory response due to its ability to enhance survival.

II. Description
Recombinant HEK293 cell expressing firefly luciferase gene under the control of NF-κB response elements with constitutive expression of human OX40 (Tumor Necrosis Factor Receptor Superfamily Member 4, TNFRSF4, and CD134, GenBank Accession No. NM_003327)

III. Introduction
Host Cell:HEK293
Expressed gene:NFκB-Luciferase-OX40
Stability:32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s):OX40, OX40 cell Line, CD134 Cell Line, ox40 nfkb
Freeze Medium:90% FBS+10% DMSO
Culture Medium:MEM+10% Foetal Bovine Serum (FBS)+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) +1ug/ml puromycin+100ug/ml hygromycin
Mycoplasma Testing:Negative
Storage:Liquid nitrogen
Application(s):Functional(Report Gene) Assay

IV. Description of Host Cell Line
Organism:Homo sapiens, human
Tissue:Embryonic kidney
Disease: Normal
Morphology: Epitheloid cell
Growth Properties: Adherent

Ⅴ. Representative Data

Figure 1. Recombinant HEK293 cell expressing firefly luciferase gene under the control of NFκB response elements with constitutive expression of human OX40 (Tumor Necrosis Factor Receptor Superfamily Member 4, TNFRSF4, and CD134, GenBank Accession No. NM_003327)


Figure 2. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly suggest to purchase from Cobioer). HEK293/NFκB Luciferase-OX40 Reporter cells were stimulated by OX40L, the EC50 was 82.7 ng/ml.




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