加入收藏 | 设为首页 | 联系我们

产品搜索

相关文章

联系我们

联系人:蒋经理
电话:86-25-86880024
号码:86-25-86880024
手机:18066071954
地址:南京市雨花区长虹路222号
Email: duhaitao@cobioer.com

产品展示 / PRODUCTS
药靶细胞株 > 肿瘤免疫细胞株 > CBP74018PD1/NFAT-Luc/Jurkat

PD1/NFAT-Luc/Jurkat
名称 PD1/NFAT-Luc/Jurkat
型号 CBP74018
报价
特点 PD1/NFAT-Luc/Jurkat,冻存条件:90% FBS+10% DMSO;
  • 详细内容

PD1/NFAT-Luc/Jurkat

CBP74018

I. Background
This PD-1 Cell Line is used to measure the binding of PD-1 to PD-L1/PD-L2. Programmed Cell Death Protein 1 (PD-1), a receptor expressed on activated T cells, binds to its ligands, PD-L1 and PD-L2, to negatively regulate immune responses. The PD-1 ligands are found on most cancers, and PD-1:PD-L1/2 interaction inhibits T cell activity and allows cancer cells to escape immune surveillance. The PD-1:PD-L1/2 pathway is also involved in regulating autoimmune responses, making these proteins promising therapeutic targets for a number of cancers, as well as multiple sclerosis, arthritis, lupus, and type I diabetes.

II. Description
Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human PD-1 (Programmed Cell Death 1, PDCD1, SLEB2, CD279, GenBank Accession #NM_005018).

III. Introduction
Host Cell:Jurkat
Expressed gene:NFAT-Luciferase-PD1
Stability:32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s):Programmed cell death 1, PDCD1, PD-1, PD1, SLEB2, CD279, HPD-L, PD1/NFAT, PD-1 NFAT, PD-1/NFAT
Freeze Medium:90% FBS+10% DMSO
Culture Medium:RPMI-1640+10%FBS+800ug/ml hygromycin+1ug/ml puromycin
Mycoplasma Testing:Negative
Storage:Liquid nitrogen
Application(s):Use this PD-1 Cell Line to screen for activators or inhibitors of PD-1 signaling in a cellular context • Characterize the biological activity of PD-1 and its interactions with ligands

IV. Description of Host Cell Line
Organism:Homo sapiens, human
Tissue:Peripheral blood
Disease: Acute T cell leukemia
Morphology: Lymphoblast
Growth Properties: Suspension

Ⅴ. Representative Data

Figure 1.Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human PD-1 (Programmed Cell Death 1, PDCD1, SLEB2, CD279, GenBank Accession #NM_005018).



Figure 2. Dose response curve of anti-PD-L1 antibody on PD-1/PD-L1 Blockade Bioassay Model. The bioassay consists of two genetically engineered cell lines, Jurkat_PD-1_NFAT-Luc Cells and CHO/TCR activitor/PD-L1 Cells. When co-cultured, the PD-1/PD-L1 interaction inhibits TCR-mediated luminescence. When the PD-1/PD-L1 interaction is disrupted, TCR activation induces luminescence (via activation of the NFAT pathway) that can be detected and quantified using Bio-Glo™; Reagent.




如果你对CBP74018PD1/NFAT-Luc/Jurkat感兴趣,想了解更详细的产品信息,填写下表直接与厂家联系:


留言框

  • 产品:

  • 您的单位:

  • 您的姓名:

  • 联系电话:

  • 常用邮箱:

  • 省份:

  • 详细地址:

  • 补充说明:

  • 验证码:

    请输入计算结果(填写阿拉伯数字),如:三加四=7
QQ在线客服
电话咨询
  • 25-86880024
  • 手机:18066071954

化工仪器网

推荐收藏该企业网站
扫一扫访问手机站扫一扫访问手机站
访问手机站