PD1/NFAT-Luc/Jurkat-南京科佰生物科技有限公司

产品搜索

产品分类

联系我们

联系人：蒋经理
电话：86-25-86880024
号码：86-25-86880024
手机：18066071954
地址：南京市雨花区长虹路222号
Email： duhaitao@cobioer.com

产品展示 / PRODUCTS
药靶细胞株 > 肿瘤免疫细胞株 > CBP74018PD1/NFAT-Luc/Jurkat

名称	PD1/NFAT-Luc/Jurkat
型号	CBP74018
报价
特点	PD1/NFAT-Luc/Jurkat，冻存条件：90% FBS+10% DMSO；

详细内容

PD1/NFAT-Luc/Jurkat CBP74018
I. Background
This PD-1 Cell Line is used to measure the binding of PD-1 to PD-L1/PD-L2. Programmed Cell Death Protein 1 (PD-1), a receptor expressed on activated T cells, binds to its ligands, PD-L1 and PD-L2, to negatively regulate immune responses. The PD-1 ligands are found on most cancers, and PD-1:PD-L1/2 interaction inhibits T cell activity and allows cancer cells to escape immune surveillance. The PD-1:PD-L1/2 pathway is also involved in regulating autoimmune responses, making these proteins promising therapeutic targets for a number of cancers, as well as multiple sclerosis, arthritis, lupus, and type I diabetes.

II. Description
Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human PD-1 (Programmed Cell Death 1, PDCD1, SLEB2, CD279, GenBank Accession #NM_005018).

III. Introduction
Host Cell:	Jurkat
Expressed gene:	NFAT-Luciferase、PD1
Stability:	32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s):	Programmed cell death 1, PDCD1, PD-1, PD1, SLEB2, CD279, HPD-L, PD1/NFAT, PD-1 NFAT, PD-1/NFAT
Freeze Medium:	90% FBS+10% DMSO
Culture Medium:	RPMI-1640+10%FBS+800ug/ml hygromycin+1ug/ml puromycin
Mycoplasma Testing:	Negative
Storage:	Liquid nitrogen
Application(s):	Functional(Report Gene) Assay

IV. Description of Host Cell Line
Organism:	Homo sapiens, human
Tissue:	Peripheral blood
Disease:	Acute T cell leukemia
Morphology:	Lymphoblast
Growth Properties:	Suspension

Ⅴ. Representative Data

Figure 1.Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human PD-1 (Programmed Cell Death 1, PDCD1, SLEB2, CD279, GenBank Accession #NM_005018).

Figure 2. Dose response curve of anti-PD-L1 antibody on PD-1/PD-L1 Blockade Bioassay Model. The bioassay consists of two genetically engineered cell lines, Jurkat_PD-1_NFAT-Luc Cells and CHO/TCR activitor/PD-L1 Cells. When co-cultured, the PD-1/PD-L1 interaction inhibits TCR-mediated luminescence. When the PD-1/PD-L1 interaction is disrupted, TCR activation induces luminescence (via activation of the NFAT pathway) that can be detected and quantified using Bio-Glo™; Reagent.
Figure 3. Dose Response of PD-1 Agonist Antibody in PD-1/NFAT-Luc/Jurkat Cells With FCγR aAPC Cell.