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药靶细胞株 > 肿瘤免疫细胞株 > CBP74020TIGIT/NFAT-Luc/Jurkat

TIGIT/NFAT-Luc/Jurkat
名称 TIGIT/NFAT-Luc/Jurkat
型号 CBP74020
报价
特点 TIGIT/NFAT-Luc/Jurkat,冻存条件:90% FBS+10% DMSO;
  • 详细内容

TIGIT/NFAT-Luc/Jurkat

CBP74020
I. Background
TIGIT is a co-inhibitory receptor that is highly expressed in Natural Killer (NK) cells, activated CD4+, CD8+ and regulatory T cells. Interaction with the poliovirus receptor (PVR; CD155) on antigen presenting cells, such as dendritic cells, recruits Src homology (SH) domain-containing protein tyrosine phosphatase SHP1 and SHP2 or the inositol phosphatase SHIP1 and SHIP2 to the TIGIT ITIM domain. This increases IL-10 release and suppresses NF-kB and NFAT T cell receptor (TCR) signaling, which blocks T cell proliferation and cytokine production. It serves as a competitive inhibitor of CD226, a co-stimulatory receptor for CD155. TIGIT targeting antibodies that block this T cell-intrinsic inhibitory effects have shown enhanced anti-tumor and anti-viral functions in preclinical studies.
II. Description

Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human TIGIT (V-set and immunoglobulin domain-containing protein 9, VSIG9, V-set and transmembrane domain-containing protein 3, and VSTM3, GenBank Accession No. NM_173799).

III. Introduction
Host Cell:Jurkat
Expressed gene:TIGIT
Stability:32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s):T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain-containing protein 9, VSIG9, V-set and transmembrane domain-containing protein 3, VSTM3
Freeze Medium:90% FBS+10% DMSO
Culture Medium:RPMI-1640+10%FBS+800ug/ml hygromycin+1ug/ml puromycin
Mycoplasma Testing:Negative
Storage:Liquid nitrogen
IV. Description of Host Cell Line
Organism:Homo sapiens, human
Tissue:Peripheral blood
Disease: Acute T cell leukemia
Morphology: Lymphoblast
Growth Properties: Suspension
Ⅴ. Representative Data

 COBIOER

Figure 1.Recombinant Jurkat cell line expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of TIGIT.

 COBIOER


Figure 2. Dose response of anti-TIGIT neutralizing antibody in TIGIT/NFAT-Reporter Jurkat cell line (C26) with CD155/TCR activator-CHO, the EC50 was 1.24μg/ml.



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