| 名称 | PD1/TIGIT Dual Effector Reporter Cell |
| 型号 | CBP74126 |
| 报价 |  |
| 特点 | PD1/TIGIT Dual Effector Reporter Cell，冻存条件：90% FBS+10% DMSO； |
产品搜索
相关文章
联系我们
联系人：蒋经理
电话：4008750250
号码：
手机：18066071954
地址：南京市栖霞区纬地路9号
Email： zhangxiangwen@cobioer.com
电话：4008750250
号码：
手机：18066071954
地址：南京市栖霞区纬地路9号
Email： zhangxiangwen@cobioer.com
产品展示 / PRODUCTS
- 详细内容
PD1/TIGIT Dual Effector Reporter Cell
| CBP74126 | |
| I. Background | |
The binding of Programmed Cell Death Protein 1 (PD-1), a receptor expressed on activated T-cells, to its ligands, PD-L1 and PD-L2, negatively regulates immune responses. PD-1 ligands are found on most cancers, and the PD-1:PD-L1/2 interaction inhibits T-cell activity and enables cancer cells to escape immune surveillance. The PD-1:PD-L1/2 pathway is also involved in regulating autoimmune responses, making these proteins promising therapeutic targets for a number of cancers, as well as multiple sclerosis, arthritis, lupus, and type I diabetes. TIGIT is a co-inhibitory receptor that is highly expressed in Natural Killer (NK) cells, activated CD4+, CD8+ and regulatory T cells. Interaction with the poliovirus receptor (PVR; CD155) on antigen presenting cells, such as dendritic cells, recruits Src homology (SH) domain-containing protein tyrosine phosphatase SHP1 and SHP2 or the inositol phosphatase SHIP1 and SHIP2 to the TIGIT ITIM domain. This increases IL-10 release and suppresses NF-kB and NFAT T cell receptor (TCR) signaling, which blocks T cell proliferation and cytokine production. It serves as a competitive inhibitor of CD226, a co-stimulatory receptor for CD155. TIGIT targeting antibodies that block this T cell-intrinsic inhibitory effects have shown enhanced anti-tumor and anti-viral functions in preclinical studies. | |
| II. Introduction | |
| Expressed gene: | PD1 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K+10%FBS+600ug/ml hygromycin+2ug/ml puromycin+5ug/ml blasticidin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| III. Representative Data | |
Figure 1. Recombinant PD1/TIGIT Dual Effector Reporter Cell constitutively expressing PD1.
Figure 2. Recombinant PD1/TIGIT Dual Effector Reporter Cell constitutively expressing TIGIT.
Figure 3. Dose Response of Blocking Antibodies in PD-1/TIGIT Dual Effector Reporter Cells (Clone 7) With PD-L1&CD155/ TCR Activator - CHO Cells. | |
