| 名称 | OVA/B16-F10 |
| 型号 | CBPG0012 |
| 报价 |  |
| 特点 | OVA/B16-F10 |
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Email： zhangxiangwen@cobioer.com
电话：4008750250
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手机：18066071954
地址：南京市栖霞区纬地路9号
Email： zhangxiangwen@cobioer.com
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- 详细内容
| I. Background | |
| OVA 基因（Ovalbumin）是鸡卵清蛋白编码基因，分子量约 43kDa，含 12269 个氨基 酸，为免疫学与肿瘤研究的核心工具。其表位如 SIINFEKL 可激活 CD8+ T 细胞，用于构 建稳转细胞系评估 CAR-T/TCR-T 疗效及疫苗免疫应答。OVA 四聚体技术可精准检测抗原 特异性 T 细胞，在肿瘤免疫监测、表观遗传调控（如 DNA 甲基化）及胚胎发育研究中发 挥关键作用，是精准免疫医学的重要实验平台。 | |
| II. Introduction | |
| Host Cell: | B16-F10 |
| Expressed gene: | OVA |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+2μg/ml Puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| III. Description of Host Cell Line | |
| Organism: | Mus musculus, mouse |
| Tissue: | skin |
| Morphology: | mixture of spindle-shaped and epithelial-like cells |
| Growth Properties: | Adherent |
| IV. Representative Data | |
Figure 1. WB of OVA/B16-F10. | |
| V. In VIVO Data | |
Index | Content |
Animal | C57BL/6J, 7weeks, male |
Implanted Position | Subcutaneous (right lower flank region ) |
Injected Cell Numbers | 2×10^5/0.1ml |
Matrix gel | Without matrix gel |
1. Weight Change
Figure 2. OVA/B16-F10 Mean Body Weight ± SEM.
2. Tumor Volume
Figure 3. OVA/B16-F10 Mean Tumor Volume ± SEM .
NOTE：The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).
