名称 | BCMA/NFkB-Luc/HEK293 |
型号 | CBP74072 |
报价 | |
特点 | BCMA/NFkB-Luc/HEK293,母细胞:HEK293,冻存条件:90% FBS+10% DMSO |
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CBP74072 | |
I. Background | |
B-Cell Maturation Antigen (BCMA), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17) or CD269, is a type I membrane protein encoded by the TNFRSF17 gene. TNFRSF17 is a cell surface receptor of the TNF receptor superfamily, that recognizes its ligands including BAFF (B-cell activating factor) and APRIL (a proliferation inducing ligand). BCMA is preferentially expressed in mature B lymphocytes and also on Multiple Myeloma (MM) cells. In vitro, the activation of BCMA by its ligand promotes the differentiation and proliferation of B cells. A vast array of intracellular activity is involved in BCMA-induced signal transduction, including activation of the NF-κB signaling pathway. The bioactivity of BAFF and APRIL as soluble homotrimers distinguishes them from other TNFSF ligands such as TRAIL, FasL, and CD40L, which are only active as membrane-bound molecules. | |
II. Description | |
The BCMA / NF-κB - HEK293 recombinant cell line has been transfected with full-length human BCMA cDNA (Genbank #NM_001192) under control of a CMV promoter for high constitutive expression. The NF-κB–luciferase reporter is also stably integrated into the genome. | |
III. Introduction | |
Host Cell: | HEK293 |
Expressed gene: | BCMA |
Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Synonym(s): | tumor necrosis factor receptor superfamily member 17, B-Cell Maturation Antigen, TNFRSF17, CD269 |
Freeze Medium: | 90% FBS+10% DMSO |
Culture Medium: | MEM + 10% Foetal Bovine Serum (FBS)+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) +100ug/ml hygromycin+1ug/ml puromycin |
Mycoplasma Testing: | Negative |
Storage: | Liquid nitrogen |
Application(s): | Functional(Report Gene) Assay |
IV. Description of Host Cell Line | |
Organism: | Human |
Tissue: | Kidney |
Morphology: | Epitheloid cell |
Growth Properties: | Adherent |
Ⅴ. Representative Data | |
Figure 1.Recombinant HEK293 cell line expressing firefly luciferase gene under the control of NFκB response elements with constitutive expression of BCMA. | |
Figure 2.Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly suggest to purchase from Cobioer).HEK293-BCMA-NFκB Luciferase Reporter cells were stimulated by rhBAFF, the EC50 was 34.6ng/ml. | |