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药靶细胞株 > 肿瘤免疫细胞株 > CBP74072BCMA/NFkB-Luc/HEK293

BCMA/NFkB-Luc/HEK293
名称 BCMA/NFkB-Luc/HEK293
型号 CBP74072
报价
特点 BCMA/NFkB-Luc/HEK293,母细胞:HEK293,冻存条件:90% FBS+10% DMSO
  • 详细内容


CBP74072
I. Background
B-Cell Maturation Antigen (BCMA), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17) or CD269, is a type I membrane protein encoded by the TNFRSF17 gene. TNFRSF17 is a cell surface receptor of the TNF receptor superfamily, that recognizes its ligands including BAFF (B-cell activating factor) and APRIL (a proliferation inducing ligand). BCMA is preferentially expressed in mature B lymphocytes and also on Multiple Myeloma (MM) cells. In vitro, the activation of BCMA by its ligand promotes the differentiation and proliferation of B cells. A vast array of intracellular activity is involved in BCMA-induced signal transduction, including activation of the NF-κB signaling pathway. The bioactivity of BAFF and APRIL as soluble homotrimers distinguishes them from other TNFSF ligands such as TRAIL, FasL, and CD40L, which are only active as membrane-bound molecules.
II. Description

The BCMA / NF-κB - HEK293 recombinant cell line has been transfected with full-length human BCMA cDNA (Genbank #NM_001192) under control of a CMV promoter for high constitutive expression. The NF-κB–luciferase reporter is also stably integrated into the genome. The firefly luciferase gene is controlled by four copies of the NF-κB Response Element upstream of a minimal promoter. Upon ligand binding, active BCMA will initiate the NF-κB signaling pathway, leading to expression of the NF-κB-controlled luciferase reporter.

III. Introduction
Host Cell:HEK293
Expressed gene:BCMA
Stability:32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s):tumor necrosis factor receptor superfamily member 17, B-Cell Maturation Antigen, TNFRSF17, CD269
Freeze Medium:90% FBS+10% DMSO
Culture Medium:MEM + 10% Foetal Bovine Serum (FBS)+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) +100ug/ml hygromycin+1ug/ml puromycin
Mycoplasma Testing:Negative
Storage:Liquid nitrogen
IV. Description of Host Cell Line
Organism:Human
Tissue:Kidney
Morphology: Epitheloid cell
Growth Properties: Adherent
Ⅴ. Representative Data

CBP74072-1.png

Figure 1.Recombinant HEK293 cell line expressing firefly luciferase gene under the control of NFκB response elements with constitutive expression of BCMA.


CBP74072-2.png

Figure 2. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. HEK293-BCMA-NFκB Luciferase Reporter cells were stimulated by rhBAFF, the EC50 was 34.6ng/ml.






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