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药靶细胞株 > 免疫治疗细胞株 > CBP74058SBE-Luc/HEK293

SBE-Luc/HEK293
名称 SBE-Luc/HEK293
型号 CBP74058
报价
特点 SBE-Luc/HEK293,冻存条件:90% FBS+10% DMSO;培养基:DMEM+10%FBS+100ug/ml hygromycin
  • 详细内容

 SBE-Luc/HEK293

CBP74058
I. Background
The transforming growth factor beta (TGFβ) signaling pathway is involved in a diverse range of cell processes such as differentiation, cell cycle arrest, and immune regulation. TGFβ signaling has been linked to cardiac disease, cancer, Alzheimer’s and other human diseases. TGFβ proteins bind to receptors on the cell surface, initiating a signaling cascade that leads to phosphorylation and activation of SMAD2 and SMAD3, which then form a complex with SMAD4. The SMAD complex then translocates to the nucleus and binds to the SMAD binding element (SBE) in the nucleus, leading to transcription and expression of TGFβ/SMAD responsive genes.
 
II. Description
The SBE Reporter-HEK293 Cell Line is designed for monitoring the activity of the TGF /SMAD signaling pathway. The transforming growth factor beta (TGF) signaling pathway is involved in a diverse range of cell processes such as differentiation, cell cycle arrest, and immune regulation. TGF signaling has been linked to cardiac disease, cancer, Alzheimer’s and other human diseases. TGF proteins bind to receptors on the cell surface, initiating a signaling cascade that leads to phosphorylation and activation of SMAD2 and SMAD3, which then form a complex with SMAD4. The SMAD complex then translocates to the nucleus and binds to the SMAD binding element (SBE) in the nucleus, leading to transcription and expression of TGF / SMAD responsive genes.
 
III. Introduction
Host Cell:HEK293
Expressed gene:SBE-Luciferase
Stability:32 passages
Synonym(s):SBE reporter, TGFβ reporter, TGFbeta reporter, SMAD reporter
Freeze Medium:90% FBS+10% DMSO
Culture Medium:DMEM+10%FBS+100ug/ml hygromycin
Mycoplasma Testing:Negative
Storage:Liquid nitrogen
Application(s):·Monitor TGF signaling pathway activity.
·Screen for activators or inhibitors of the TGF/SMAD signaling pathway.
 
IV. Description of Host Cell Line
Organism:Homo sapiens, human
Tissue:Embryonic kidney
Disease: Normal
Morphology: Epitheloid cell
Growth Properties: Adherent
 
Ⅴ. Representative Data

 


 


Figure . Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. HEK293/SBE Luciferase Reporter cells were stimulated by TGF, the S/B was 138.3-fold.

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