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药靶细胞株 > 肿瘤免疫细胞株 > CBP74076mouse ADCC Bioassay Effector Cell Line

mouse ADCC Bioassay Effector  Cell Line
名称 mouse ADCC Bioassay Effector Cell Line
型号 CBP74076
报价
特点 Mouse ADCC Bioassay Effector Cell Line,冻存条件:90% FBS+10% DMSO
  • 详细内容

mouse ADCC Bioassay Effector  Cell Line

CBP74076                
I. Background                

Antibody-dependent cell-mediated cytotoxicity (ADCC) is an immune defense mechanism involving an effector cell lysing a target cell on which antibodies have bound to specific antigens on the target cell membrane.                    

The typical ADCC involves activation of natural killer (NK) cells by antibodies. NK cells express Fc receptors, mostly CD16 or FcγRIIIa (CD16a), on its cell surface. These Fc receptors recognize and bind to the Fc portion of an antibody, such as IgG, which has bound to the surface of a pathogen-infected target cell. Once the Fc receptor binds to the Fc region of IgG, the Natural Killer cell releases cytokines such as IFN-γ and cytotoxic molecules that attack the pathogen-infected target cell.                    

The human FcγRIIIa displays a dimorphism in the position of residue 158. One allele (V158) encodes a higher Fc affinity receptor variant with a valine at amino acid residue 158, and the other (F158) encodes a lower Fc affinity receptor variant having a phenylalanine at amino acid residue 158.                    

               
II. Description                

Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human FcγRIIIa, low affinity (F158) variant and Fcγ chain.                    

                   

 COBIOER                    

               
III. Introduction                
Host Cell:                Jurkat                
Expressed gene:                NFAT                
Stability:                32 passages  (in-house test, that not means the cell line will be instable beyond the passages we tested.)               
Synonym(s):                Antibody-dependent cell-mediated cytotoxicity                
Freeze Medium:                90% FBS+10% DMSO                
Culture Medium:                RPMI-1640+10%FBS+800ug/ml hygromycin+1ug/ml puromycin            
Mycoplasma Testing:                Negative                
Storage:                Immediately upon receipt, store in liquid nitrogen.                
Application(s):                Characterize the Fc effector function of antibodies and measure ADCC activity in cellular assays.                
               
IV. Description of Host Cell Line                
Organism:                Homo sapiens, human                
Tissue:                Peripheral blood                
Disease:                Acute T cell leukemia                
Morphology:                Lymphoblast                
Growth Properties:                Suspension                
               
Ⅴ. Representative Data                
 COBIOER                



Figure 8.  Dose response of mouse Anti-CD20 lgG2a in Mouse ADCC Bioassay Effector Cell (C3) .                    






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