EDNRB/β-Arrestin/CHO GPCR细胞-南京科佰生物科技有限公司

产品搜索

联系人：蒋经理
电话：4008750250
号码：
手机：18066071954
地址：南京市栖霞区纬地路9号
Email： zhangxiangwen@cobioer.com

CBP71376
I. Background
EDNRB (Endothelin Receptor Type B) is a Protein Coding gene. Diseases associated with EDNRB include Waardenburg Syndrome, Type 4A and Abcd Syndrome. Among its related pathways are Class A/1 (Rhodopsin-like receptors) and Endothelins. Gene Ontology (GO) annotations related to this gene include G protein-coupled receptor activity and type 1 angiotensin receptor binding.

II. Introduction
Host Cell:	CHO
Stability:	20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium:	90% FBS+10% DMSO
Culture Medium:	F12K+10%FBS+5ug/ml puromycin+5ug/ml blasticidin
Mycoplasma Status:	Negative
Storage:	Liquid nitrogen immediately upon delivery
Application(s):	Functional assay for EDNRB receptor
Transducer:	Activation of ETB receptors to produce a range of biological actions in different tissues is thought to be mediated by a number of transduction systems coupled to various types of G-protein including Gq/G11, Gs, Gq/13 and Gi2.

Ⅲ. Description of Host Cell Line
Organism:	Hamster
Tissue:	Ovary
Morphology:	Epithelial
Growth Properties:	Adherent

Ⅳ. Representative Data
Figure 1.Recombinant EDNRB/β-Arrestin/CHO constitutively expressing EDNRB/β-Arrestin.
Figure 2. Dose response of Endothelin 3 (human,mouse,rabbit, rat TFA) in CHO-K1 EDNRB(ET2,ETB) β-Arrestin Cell line（C11）.