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药靶细胞株 > GPCR细胞株 > CBP71391CB1/β-Arrestin/CHO GPCR细胞

CB1/β-Arrestin/CHO  GPCR细胞
名称 CB1/β-Arrestin/CHO GPCR细胞
型号 CBP71391
报价
特点 CB1/β-Arrestin/CHO
  • 详细内容
CBP71391
I. Background
The cannabinoid receptor CNR1 is Gs-coupled GPCRs expressed in primarily CNS and some peripheral neurones; particularly prevalent in basal ganglia, hippocampus, cerebellum, cerebral cortex and also presented in some non-neuronal cells and tissues, for example leukocytes and testis. A shorter human splice variant (411aa) has been identified in the brain and other tissues by reverse-transcriptase PCR but mRNA levels were less than 10-fold the levels of the longer isoform. The pharmacological characteristics of the isoforms are similar.
 
II. Introduction
Host Cell:

CHO

Stability:20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium:90% FBS+10% DMSO
Culture Medium:F12k+10%FBS+5ug/ml puromycin+5ug/ml blasticidin
Mycoplasma Status:Negative
Storage:Liquid nitrogen immediately upon delivery
Application(s):Functional assay for CB1 receptor
Transducer:Gi/Go family
 
Ⅲ. Description of Host Cell Line
Organism:Hamster
Tissue:Ovary
Morphology: Epithelial
Growth Properties: Adherent
 
Ⅳ. Representative Data

CBP71391 流式.png

Figure 1. Recombinant CB1/β-Arrestin/CHO constitutively expressing CB1.

CBP71391 数据图1.jpg

Figure 2.Dose response of CP-55940 in CB1 β-Arrestin CHO-K1 Cell Line (C2)

CBP71391 数据图2.jpg


Figure 3.Inhibition of CP-55940-induced Beta-Arrestin Recruitment in CB1 Beta-Arrestin CHO-K1 Cells (C2).

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