CCR5/β-Arrestin/CHO GPCR细胞-南京科佰生物科技有限公司

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CBP71392
I. Background
C-C chemokine receptor type 5 (CCR5) is a G protein-coupled receptor and a co-receptor for the entry of human immunodeficiency virus-1 (HIV-1) into cells. CCR5 chemokine receptor is involved in leucocytes chemotaxis to sites of inflammation and plays an important role in the macrophages, T cells, and monocytes recruitment. The chemokine ligands that bind to CCR5 are regulated on activation, normal T Cell expressed and secreted (RANTES) and macrophage inflammatory protein 1 alpha (MIP1α). GenScript’s human CCR5-expressing stable subline is guaranteed to function properly in the calcium flux assay.

II. Introduction
Host Cell:	CHO
Stability:	20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium:	90% FBS+10% DMSO
Culture Medium:	F12k+10%FBS+5ug/ml puromycin+5ug/ml blasticidin
Mycoplasma Status:	Negative
Storage:	Liquid nitrogen immediately upon delivery
Application(s):	Functional assay for CCR5 receptor
Transducer:	Gi/Go family

Ⅲ. Description of Host Cell Line
Organism:	Hamster
Tissue:	Ovary
Morphology:	Epithelial
Growth Properties:	Adherent

Ⅳ. Representative Data
Figure 1. Dose response of Human CCL3 in CCR5 B-Arrestin CHO-K1 Cell Line (C18).
Figure 2. Inhibition of hCCL3-induced Beta-Arrestin Recruitment in CCR5 Beta-Arrestin CHO-K1 Cells (C18).