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药靶细胞株 > GPCR细胞株 > CBP71392CCR5/β-Arrestin/CHO GPCR细胞

CCR5/β-Arrestin/CHO  GPCR细胞
名称 CCR5/β-Arrestin/CHO GPCR细胞
型号 CBP71392
报价
特点 CCR5/β-Arrestin/CHO
  • 详细内容
CBP71392
I. Background
C-C chemokine receptor type 5 (CCR5) is a G protein-coupled receptor and a co-receptor for the entry of human 
immunodeficiency virus-1 (HIV-1) into cells. CCR5 chemokine receptor is involved in leucocytes chemotaxis to sites of 
inflammation and plays an important role in the macrophages, T cells, and monocytes recruitment. The chemokine 
ligands that bind to CCR5 are regulated on activation, normal T Cell expressed and secreted (RANTES) and macrophage inflammatory protein 1 alpha (MIP1α). GenScript’s human CCR5-expressing stable subline is guaranteed to function properly in the calcium flux assay.
 
II. Introduction
Host Cell:

CHO

Stability:20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium:90% FBS+10% DMSO
Culture Medium:F12k+10%FBS+5ug/ml puromycin+5ug/ml blasticidin
Mycoplasma Status:Negative
Storage:Liquid nitrogen immediately upon delivery
Application(s):

Functional assay for CCR5 receptor

Transducer:Gi/Go family
 
Ⅲ. Description of Host Cell Line
Organism:Hamster
Tissue:Ovary
Morphology: Epithelial
Growth Properties: Adherent
 
Ⅳ. Representative Data

CBP71392 数据图1.jpg

Figure 1. Dose response of Human CCL3 in CCR5 B-Arrestin CHO-K1 Cell Line (C18).

CBP71392 数据图2.jpg


Figure 2. Inhibition of hCCL3-induced Beta-Arrestin Recruitment in CCR5 Beta-Arrestin CHO-K1 Cells (C18).

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