| 名称 | ADCC Bioassay Effector Cell F variant (Low Affinit |
| 型号 | CBP74626 |
| 报价 |  |
| 特点 | ADCC Bioassay Effector Cell F variant (Low Affinity)- Fcγ-NFAT Jurkat(CD3E KO) |
产品搜索
相关文章
联系我们
联系人：蒋经理
电话：4008750250
号码：
手机：18066071954
地址：南京市栖霞区纬地路9号
Email： zhangxiangwen@cobioer.com
电话：4008750250
号码：
手机：18066071954
地址：南京市栖霞区纬地路9号
Email： zhangxiangwen@cobioer.com
产品展示 / PRODUCTS
- 详细内容
ADCC Bioassay Effector Cell F variant (Low Affinity)- Fcγ-NFAT Jurkat(CD3E KO)
| I. Background | |
抗体依赖的细胞介导的细胞毒性作用（ADCC，antibody-dependent cell-mediated cytotoxicity）是指抗体的Fab 段结合病毒感染的细胞或肿瘤细胞的抗原表位，其Fc段与杀伤细胞（NK 细胞、巨噬细胞等）表面的FcR 结合，介导杀伤细胞直接杀伤靶细胞，它是抗肿瘤的治疗性抗体药物发生作用的一种作用重要机制。 | |
| II. Description | |
ADCC Bioassay Effector Cell F variant (Low Affinity)-Fcγ-NFAT Jurkat(CD3E KO)报告基 因药靶模型很好的模拟了体内ADCC 的信号转导过程，原理见下图所示。
| |
| III. Introduction | |
| Host Cell: | Jurkat |
| Expressed gene: | NFAT-Luc-CD16a(F158) |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS +400 μg/ml Hygromycin B+1 μg/ml Puromycin+10 μg/ml Blasticidin |
| Mycoplasma Testing: | Negative |
| Storage: | Immediately upon receipt, store in liquid nitrogen. |
| Application(s): | Functional(Report Gene) Assay |
| IV. Description of Host Cell Line | |
| Organism: | Homo sapiens, human |
| Tissue: | Peripheral blood |
| Disease: | Acute T cell leukemia |
| Morphology: | Lymphoblast |
| Growth Properties: | Suspension |
| Ⅴ. Representative Data | |
Figure 2. ADCC Bioassay Effector Cell F variant (Low Affinity)- Fcγ-NFAT Jurkat(CD3E KO) 细胞流式验证 CD3E 的表达。
Figure 3. ADCC Bioassay Effector Cell F variant (Low Affinity)- Fcγ-NFAT Jurkat(CD3E KO) 细胞流式验证 CD16A 的表达。
Figure 4. CD3E(NM_000733.4):c.397_398ins4bp/CD3E:p.I133Kfs
Figure 5. Dose Response of CD3E Ab in ADCC Bioassay Effector Cell F variant (Low Affinity)-Fcγ-NFAT Jurkat(CD3E KO,C22).
