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药靶细胞株 > kinase激酶细胞株 > CBP73209ETV6-NTRK3 G696A/BaF3

名称	ETV6-NTRK3 G696A/BaF3
型号	CBP73209
报价
特点	ETV6-NTRK3 [G696A]/BaF3,母细胞：BaF3，冻存条件：90% FBS+10% DMSO

详细内容

CBP73209
I. Introduction
Cell Line Name：	ETV6-NTRK3 [G696A]/BaF3
Host Cell:	BA/F3
Stability:	16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Application:	Anti-proliferation assay and PD assay
Freeze Medium:	90% FBS+10% DMSO
Complete Culture Medium:	RPMI-1640+10% FBS+2 ug/ml puromycin
Mycoplasma Status:	Negative

II.Background
ETV6-NTRK3 gene fusion is the translocation of genetic material between the ETV6 gene located on the short arm (designated p) of chromosome 12 at position p13.2 (i.e. 12p13.2) and the NTRK3 gene located on the long arm (designated q) of chromosome 15 at position q25.3 (i.e. 15q25.3) to create the (12;15)(p13;q25) fusion gene, ETV6-NTRK3. This new gene consists of the 5' end of ETV6 fused to the 3' end of NTRK3. ETV6-NTRK3 therefore codes for an chimeric oncoprotein consisting of the helix-loop-helix (HLH) protein dimerization domain of the ETV6 protein fused to the tyrosine kinase (i.e. PTK) domain of the NTRK3 protein. The ETV6 gene codes for the transcription factor protein, ETV6, which suppresses the expression of, and thereby regulates, various genes that in mice are required for normal hematopoiesis as well as the development and maintenance of the vascular network. NTRK3 codes for Tropomyosin receptor kinase C a NT-3 growth factor receptor cell surface protein that when bound to its growth factor ligand, neurotrophin-3, becomes an active tyrosine kinase that phosphorylates tyrosine residues on, and thereby stimulates, signaling proteins that promote the growth, survival, and proliferation of their parent cells. The tyrosine kinase of the ETV6-NTRK3 fusion protein is dysfunctional in that it is continuously active in phophorylating tyrosine residues on, and thereby continuously stimulating, proteins that promote the growth, survival, and proliferation of their parent cells. In consequence, these cells take on malignant characteristics and are on the pathway of becoming cancerous. Indeed, the ETV6-NTRK3 fusion gene appears to be a critical driver of several types of cancers. It was originally identified in congenital fibrosarcoma and subsequently found in secretory breast cancer (also termed juvenile breast cancer), Mammary analogue secretory carcinoma of salivary glands (also termed MASC or MASCSG), congenital fibrosarcoma, congenital mesoblastic nephroma, rare cases of acute myelogenous leukemia, ALK-negative Inflammatory myofibroblastic tumour, and radiation-induced papillary thyroid carcinoma.

III. Representative Data
1. WB of ETV6-NTRK3-G696A/BaF3 expression


2. Sanger of ETV6-NTRK3 [G696A]/BaF3 Figure 2. SNP of ETV6-NTRK3-G696A/BaF3 Figure 3. Breakpoint of ETV6-NTRK3-G696A/BaF3
3. Anti-proliferation assay

Figure 4. Anti-proliferation assay of two reference compounds on the ETV6-NTRK3-G696A/BaF3 Stable Cell Line