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药靶细胞株 > 肿瘤免疫细胞株 > CBP74061OX40/NFkB-Luc/Jurkat

OX40/NFkB-Luc/Jurkat
名称 OX40/NFkB-Luc/Jurkat
型号 CBP74061
报价
特点 OX40/NFkB-Luc/Jurkat,冻存条件:90% FBS+10% DMSO;
  • 详细内容

OX40/NFkB-Luc/Jurkat

CBP74061
I. Background

Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40, is a member of the TNFR-superfamily of receptors. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation.

II. Description
Recombinant Jurkat cell expressing firefly luciferase gene under the control of NF-κB response elements with constitutive expression of human OX40 (Tumor Necrosis Factor Receptor Superfamily Member 4, TNFRSF4, and CD134, GenBank Accession No. NM_003327)
III. Introduction
Host Cell:Jurkat
Expressed gene:

OX40-NFkB-Luciferase

Stability:32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s):OX40, OX40 cell Line, CD134 Cell Line, ox40 nfkb
Freeze Medium:90% FBS+10% DMSO
Culture Medium:RPMI-1640+10%FBS+400ug/ml hygromycin+1ug/ml puromycin
Mycoplasma Testing:Negative
Storage:Liquid nitrogen
Application(s):

Functional(Report Gene) Assay

IV. Description of Host Cell Line
Organism:Human
Tissue:Peripheral blood
Disease: Childhood T acute lymphoblastic leukemia
Morphology: Lymphoblast
Growth Properties: Suspension
Ⅴ. Representative Data

 COBIOER

Figure 1. Recombinant Jurkat stably expressing human OX40.

 COBIOER

Figure 2.  Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly suggest to purchase from Cobioer). Jurkat-OX40-NFkB-Luciferase Reporter cells were stimulated by OX40L,the S/B was 5.8-fold.






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