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药靶细胞株 > 免疫治疗细胞株 > CBP74061OX40/NFkB-Luc/Jurkat

OX40/NFkB-Luc/Jurkat
名称 OX40/NFkB-Luc/Jurkat
型号 CBP74061
报价
特点 OX40/NFkB-Luc/Jurkat,冻存条件:90% FBS+10% DMSO;
  • 详细内容

 OX40/NFkB-Luc/Jurkat

CBP74061
I. Background

Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40, is a member of the TNFR-superfamily of receptors. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation.

 
II. Description
Recombinant HEK293 cell expressing firefly luciferase gene under the control of NF-κB response elements with constitutive expression of human OX40 (Tumor Necrosis Factor Receptor Superfamily Member 4, TNFRSF4, and CD134, GenBank Accession No. NM_003327)
 
III. Introduction
Host Cell:Jurkat
Expressed gene:

OX40-NFkB-Luciferase

Stability:32 passages
Synonym(s):OX40, OX40 cell Line, CD134 Cell Line, ox40 nfkb
Freeze Medium:90% FBS+10% DMSO
Culture Medium: 
Mycoplasma Testing:Negative
Storage:Liquid nitrogen
Application(s):

Screen for activators or inhibitors of OX40 signaling in a cellular context and characterize the biological activity of OX40 and its interactions with ligands

 
IV. Description of Host Cell Line
Organism:Human
Tissue:Peripheral blood
Disease: Childhood T acute lymphoblastic leukemia
Morphology: Lymphoblast
Growth Properties: Suspension
 
Ⅴ. Representative Data

 

Figure 1. Recombinant Jurkat stably expressing human OX40-NFkB.

 

Figure 2.  Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. Jurkat-OX40-NFkB-Luciferase Reporter cells were stimulated by Jurkat cells,the S/B was 5.8ug/ml.

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