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药靶细胞株 > 肿瘤免疫细胞株 > CBP74065PDL2/TCR Activator/CHO

PDL2/TCR Activator/CHO
名称 PDL2/TCR Activator/CHO
型号 CBP74065
报价
特点 PDL2/TCR Activator/CHO,冻存条件:90% FBS+10% DMSO;
  • 详细内容

PDL2/TCR Activator/CHO

CBP74065
I. Background
The binding of Programmed Cell Death Protein 1 (PD-1), a receptor expressed on activated T-cells, to its ligands, PD-L1 and PD-L2, negatively regulates immune responses. PD-1 ligands are found on most cancers, and the PD-1:PD-L1/2 interaction inhibits T-cell activity and allows cancer cells to escape immune surveillance. The PD-1:PD-L1/2 pathway is also involved in regulating autoimmune responses, making these proteins promising therapeutic targets for a number of cancers, as well as multiple sclerosis, arthritis, lupus, and type I diabetes.
II. Description
Recombinant CHO-K1 cells constitutively expressing human PD-L2 (Programmed Cell Death 1 Ligand 2 or CD273, GenBank accession #NM_025239) and an engineered T-cell receptor (TCR) activator.
III. Introduction
Host Cell:CHO-K1
Expressed gene:PD-L2
Stability:32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s):Programmed Cell Death 2 Ligand 2, PDL2, PD-L2, CD273, PDCDL1G2, B7-DC
Freeze Medium:90% FBS+10% DMSO
Culture Medium:F12K+10%FBS+2ug/ml puromycin+500ug/ml hygromycin
Mycoplasma Testing:Negative
Storage:Liquid nitrogen
Application(s):Screen for activators or inhibitors of PD-1 signaling in a cellular context
IV. Description of Host Cell Line
Organism:Cricetulus griseus, hamster, Chinese
Tissue:Ovary
Disease: Hamster Chinese ovary
Morphology: Epitheloid cell
Growth Properties: Adherent
Ⅴ. Representative Data


Figure 1.Recombinant CHO-K1 cells constitutively expressing human PD-L2 (Programmed Cell Death 1 Ligand 2 or CD273, GenBank accession #NM_025239) and an engineered T-cell receptor (TCR) activator.

Figure 2. Dose responese curve of anti-PD-1 neutralizing antibody in PD-1/NFAT-Reporter/Jurkat cells with PD-L2/TCR activator/CHO.The bioassay consists of two genetically engineered cell lines, Jurkat/PD-1/NFAT-Luc Cells and CHO/TCR activator/PD-L2 Cells. When co-cultured, the PD-1/PD-L2 interaction inhibits TCR-mediated luminescence. When the PD-1/PD-L2 interaction is disrupted, TCR activation induced luminescence (via activation of NFAT pathway) that can be detected and quantified using Bio-Glo Reagents



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