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药靶细胞株 > 免疫治疗细胞株 > CBP74066PDL1/TCR Activator/CHO

PDL1/TCR Activator/CHO
名称 PDL1/TCR Activator/CHO
型号 CBP74066
报价
特点 PDL1/TCR Activator/CHO,冻存条件:90% FBS+10% DMSO;培养基:F12K+10%FBS+2ug/ml puromycin+500ug/ml hygromycin
  • 详细内容

 PDL1/TCR Activator/CHO

CBP74066
I. Background
The binding of Programmed Cell Death Protein 1 (PD-1), a receptor expressed on activated T-cells, to its ligands, PD-L1 and PD-L2, negatively regulates immune responses. PD-1 ligands are found on most cancers, and the PD-1:PD-L1/2 interaction inhibits T-cell activity and allows cancer cells to escape immune surveillance. The PD-1:PD-L1/2 pathway is also involved in regulating autoimmune responses, making these proteins promising therapeutic targets for a number of cancers, as well as multiple sclerosis, arthritis, lupus, and type I diabetes.
 
II. Description

Recombinant CHO-K1 cells constitutively expressing human PD-L1 (Programmed Cell Death 1 Ligand 1, CD274, B7 homolog 1 (B7- H1), GenBank accession # NM_014143) an engineered T cell receptor (TCR) activator.

 
III. Introduction
Host Cell:CHO-K1
Expressed gene:PD-L1
Stability:32 passages
Synonym(s):Programmed Cell Death 1 Ligand 1, PDL1, PD-L1, B7 homolog 1, B7H1, B7-H1, CD274, PDCD1L1
Freeze Medium:90% FBS+10% DMSO
Culture Medium:F12K+10%FBS+2ug/ml puromycin+500ug/ml hygromycin
Mycoplasma Testing:Negative
Storage:Liquid nitrogen
Application(s):Screen for activators or inhibitors of PD-1 signaling in a cellular context , screen PD-L1 antibodies for binding affinity • Characterize the biological activity of PD-1 interactions with PD-L1
 
IV. Description of Host Cell Line
Organism:Cricetulus griseus, hamster, Chinese
Tissue:Ovary
Disease: Hamster Chinese ovary
Morphology: Epitheloid cell
Growth Properties: Adherent
 
Ⅴ. Representative Data

 

Figure 1.Recombinant CHO-K1 cells constitutively expressing human PD-L1 and an engineered T-cell receptor (TCR) activator.

 

Figure 2. Dose response curve of anti-PD-1 antibody on PD-1/PD-L1 Blockade Bioassay Model. The bioassay consists of two genetically engineered cell lines, Jurkat_PD-1_NFAT-Luc Cells and CHO/TCR activitor/PD-L1 Cells. When co-cultured, the PD-1/PD-L1 interaction inhibits TCR-mediated luminescence. When the PD-1/PD-L1 interaction is disrupted, TCR activation induces luminescence (via activation of the NFAT pathway) that can be detected and quantified using Bio-Glo™ Reagent.

 

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