名称 | ISRE-Luc/NFkB-SEAP/THP-1 |
型号 | CBP74097 |
报价 | ![]() |
特点 | ISRE-Luc/NFkB-SEAP/THP-1,冻存条件:90% FBS+10% DMSO; |
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- 详细内容
ISRE-Luc/NFkB-SEAP/THP-1
CBP74097 | |
I. Background | |
THP1 reporter cells are derived from THP-1, a human monocytic cell line that naturally expresses many pattern-recognition receptors, including Toll-like receptors. | |
II. Description | |
The NF-κB reporter (Luc)-THP-1 cell line is designed for monitoring nuclear factor Kappa B (NF-κB) signal transduction pathways. It contains a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. | |
III. Introduction | |
Host Cell: | THP-1 |
Expressed gene: | ISRE |
Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Synonym(s): | NF-kB reporter, NFkappaB reporter, luciferase reporter, NF-kB reporter, NFkB reporter, nfkb thp1, thp1, NF-kB, nfkb, nf-kb |
Freeze Medium: | 90% FBS+10% DMSO |
Culture Medium: | RPMI-1640+10%FBS+10 µg/ml of blasticidin+ 100 μg/ml of Zeocin |
Mycoplasma Testing: | Negative |
Storage: | Liquid nitrogen |
Application(s): | Functional(Report Gene) Assay |
IV. Description of Host Cell Line | |
Organism: | Homo sapiens, human |
Tissue: | Peripheral blood |
Disease: | Acute monocytic leukemia |
Morphology: | Monocyte |
Growth Properties: | Suspension |
Ⅴ. Representative Data | |
Figure 1. Dose Response of 2'3-cGAMP in THP-1 ISRE-Luc/NFkB-SEAP Cells , the EC50 was 13.5μM. | |