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药靶细胞株 > 肿瘤免疫细胞株 > CBP74097ISRE-Luc/NFkB-SEAP/THP-1

ISRE-Luc/NFkB-SEAP/THP-1
名称 ISRE-Luc/NFkB-SEAP/THP-1
型号 CBP74097
报价
特点 ISRE-Luc/NFkB-SEAP/THP-1,冻存条件:90% FBS+10% DMSO;
  • 详细内容

ISRE-Luc/NFkB-SEAP/THP-1

CBP74097
I. Background

THP1 reporter cells are derived from THP-1, a human monocytic cell line that naturally expresses many pattern-recognition receptors, including Toll-like receptors.

II. Description

The NF-κB reporter (Luc)-THP-1 cell line is designed for monitoring nuclear factor Kappa B (NF-κB) signal transduction pathways. It contains a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene.

III. Introduction
Host Cell:THP-1
Expressed gene:ISRE
Stability:32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s):NF-kB reporter, NFkappaB reporter, luciferase reporter, NF-kB reporter, NFkB reporter, nfkb thp1, thp1, NF-kB, nfkb, nf-kb
Freeze Medium:90% FBS+10% DMSO
Culture Medium:RPMI-1640+10%FBS+10 µg/ml of blasticidin+ 100 μg/ml of Zeocin
Mycoplasma Testing:Negative
Storage:Liquid nitrogen
Application(s):Functional(Report Gene) Assay
IV. Description of Host Cell Line
Organism:Homo sapiens, human
Tissue:Peripheral blood
Disease: Acute monocytic leukemia
Morphology: Monocyte
Growth Properties: Suspension
Ⅴ. Representative Data

CBP74097 数据图1.jpg


Figure 1. diABZI on THP1-Dual NFkB-SEAP Activity.

CBP74097 数据图2.jpg

Figure 2. diABZI on THP1-Dual ISRE-Luc Activity.






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