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药靶细胞株 > kinase激酶细胞株 > CBP73048EGFR L858R-T790M/BaF3

EGFR L858R-T790M/BaF3
名称 EGFR L858R-T790M/BaF3
型号 CBP73048
报价
特点 EGFR L858R-T790M/BaF3,母细胞:BaF3,冻存条件:90% FBS+10% DMSO,培养基:RPMI-1640+10% FBS+1 ug/ml puromycin
  • 详细内容
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CBP73048
I. Introduction

Cell Line Name:

EGFR L858R-T790M/BaF3

Gene Synonyms:

Epidermal Growth Factor Receptor, ERBB1, EGFR

Host Cell:

Ba/F3

Stability:16 passages

Application:

Anti-proliferation assay and PD assay

Freeze Medium:

90% FBS+10% DMSO

Complete Culture Medium:

RPMI-1640+10% FBS+1 ug/ml puromycin

Mycoplasma Status:

Negative

 
II.Background

EGFR is widely recognized for its importance in cancer. Amplification and mutations have been shown to be driving events in many cancer types. Its role in non-small cell lung cancer, glioblastoma and basal-like breast cancers has spurred many research and drug development efforts. Tyrosine kinase inhibitors have shown efficacy in EGFR amplfied tumors, most notably gefitinib and erlotinib. Mutations in EGFR have been shown to confer resistance to these drugs, particularly the variant T790M, which has been functionally characterized as a resistance marker for both of these drugs. The later generation TKI's have seen some success in treating these resistant cases, and targeted sequencing of the EGFR locus has become a common practice in treatment of non-small cell lung cancer. Overproduction of ligands is another possible mechanism of activation of EGFR. ERBB ligands include EGF, TGF-a, AREG, EPG, BTC, HB-EGF, EPR and NRG1-4 (for detailed information please refer to the respective ligand section). In ligand-activated cancers, Cetuximab appears to be more effective than tyrosine-kinase inhibitors.

 
III. Representative Data

1. WB of EGFR-L858R/T790M expression

CBP73048 WB.png

Figure 1. WB of EGFR Expression
Lane 1: Negative control
Lane 2: EGFR-WT
Lane 3: EGFR-L858R/T790M
Lane 4: EGFR-L858R/T790M/C797S

2. Sanger sequencing

CBP73048 sanger.png

Figure 2. Sanger Sequencing of EGFR-L858R/T790M

3. Anti-proliferation assay

CBP73048 数据图.png


Figure 3. Anti-proliferation assay of three reference compounds on the EGFR L858R-T790M/BaF3 Stable Cell Line

 

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