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药靶细胞株 > 肿瘤免疫细胞株 > CBP74030SRE-Luc/HEK293

SRE-Luc/HEK293
名称 SRE-Luc/HEK293
型号 CBP74030
报价
特点 SRE-Luc/HEK293,冻存条件:90% FBS+10% DMSO;
  • 详细内容
CBP74030
I. Background
The MAPK/ERK signaling pathway is a major participant in the regulation of cell growth and differentiation. It can be activated by various extracellular stimuli including mitogens, growth factors, and cytokines. Upon stimulation, MEK1/2 phosphorylate and activate ERK1/2. The activated ERK translocates to the nucleus where it phosphorylates and activates transcription factors. The TCFs (Ternary Complex Factors), including Elk1, are among the best-characterized transcription factor substrates of ERK. When phosphorylated by ERK, Elk1 forms a complex with Serum Response Factor (SRF) and binds to Serum Response Element (SRE), resulting in the expression of numerous mitogen-inducible genes.

II. Description
The SRE Reporter -HEK293 cell line is designed for monitoring the activity of the MAPK/ERK signaling pathway. The SRE Reporter -HEK293 cell line contains a firefly luciferase gene under the control of SRE responsive elements stably integrated into HEK293 cells, resulting in an ERK pathway-responsive reporter cell line.

III. Introduction
Host Cell:HEK293
Expressed gene:SRE-Luciferase
Stability:32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s):SRE cell line, MAPK reporter, ERK reporter
Freeze Medium:90% FBS+10% DMSO
Culture Medium:DMEM+10%FBS+200ug/ml hygromycin
Mycoplasma Testing:Negative
Storage:Liquid nitrogen
Application(s):• Monitor MAPK/ERK signaling pathway activity and SRF-mediated activity.

• Screen for activators or inhibitors of the MAPK/ERK signaling pathway.


IV. Description of Host Cell Line
Organism:Homo sapiens, human
Tissue:Embryonic kidney
Disease: Normal
Morphology: Epitheloid cell
Growth Properties: Adherent

Ⅴ. Representative Data

CBP74030.png

Figure 1. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. HEK293/SRE Luciferase Reporter cells were stimulated by EGF, the S/B was 10-fold.


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