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药靶细胞株 > 肿瘤免疫细胞株 > CBP74030SRE-Luc/HEK293

SRE-Luc/HEK293
名称 SRE-Luc/HEK293
型号 CBP74030
报价 50000
特点 SRE-Luc/HEK293,冻存条件:90% FBS+10% DMSO;
  • 详细内容
CBP74030
I. Background
The MAPK/ERK signaling pathway is a major participant in the regulation of cell growth and differentiation. It can be activated by various extracellular stimuli including mitogens, growth factors, and cytokines. Upon stimulation, MEK1/2 phosphorylate and activate ERK1/2. The activated ERK translocates to the nucleus where it phosphorylates and activates transcription factors. The TCFs (Ternary Complex Factors), including Elk1, are among the best-characterized transcription factor substrates of ERK. When phosphorylated by ERK, Elk1 forms a complex with Serum Response Factor (SRF) and binds to Serum Response Element (SRE), resulting in the expression of numerous mitogen-inducible genes.

II. Description
The SRE Reporter -HEK293 cell line is designed for monitoring the activity of the MAPK/ERK signaling pathway. The SRE Reporter -HEK293 cell line contains a firefly luciferase gene under the control of SRE responsive elements stably integrated into HEK293 cells, resulting in an ERK pathway-responsive reporter cell line.

III. Introduction
Host Cell:HEK293
Expressed gene:SRE-Luciferase
Stability:32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s):SRE cell line, MAPK reporter, ERK reporter
Freeze Medium:90% FBS+10% DMSO
Culture Medium:DMEM+10%FBS+200ug/ml hygromycin
Mycoplasma Testing:Negative
Storage:Liquid nitrogen
Application(s):Functional(Report Gene) Assay


IV. Description of Host Cell Line
Organism:Homo sapiens, human
Tissue:Embryonic kidney
Disease: Normal
Morphology: Epitheloid cell
Growth Properties: Adherent

Ⅴ. Representative Data

CBP74030 替换新.jpg


Figure 1. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly suggest to purchase from Cobioer). Dose Response of Recombinant Human EGF in SRE/Luc HEK293 Cells (C5C16).





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